List of restriction enzyme cutting sites: S

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Restriction enzymes starting with S: a concise guide

This page lists the most studied restriction enzymes whose names begin with the letter S. There are about 130 enzymes covered. For each enzyme, you’ll find the essential details to help you plan experiments:

- Recognition sequence: the DNA letters the enzyme recognizes
- Cutting pattern: whether the cut results in blunt ends or sticky (overhanging) ends
- Cleavage location: exactly where the DNA is cut relative to the recognition site
- Source organism: where the enzyme comes from
- Typical reaction conditions: common temperature and buffer
- Special notes: cofactors, related enzymes (isoschizomers), or any unusual behavior

How to use this list: pick an enzyme based on the target DNA sequence, the type of ends you need for cloning, and the lab conditions you can meet. The entries are organized to help you compare options quickly and plan your experiments efficiently.