2001 Czech Republic motorcycle Grand Prix

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The 2001 Czech Republic motorcycle Grand Prix was the 10th round of the 2001 season. It took place at the Masaryk Circuit in Brno on the weekend of 24–26 August 2001.

500cc
- Pole position: Max Biaggi, 2:00.347
- Fastest lap: Valentino Rossi, 2:01.461 on lap 7
- Podium: 1) Valentino Rossi 2) Àlex Crivillé 3) Loris Capirossi

250cc
- Pole position: Tetsuya Harada, 2:02.953
- Fastest lap: Marco Melandri, 2:03.836 on lap 5
- Podium: 1) Tetsuya Harada 2) Marco Melandri 3) Daijiro Kato

125cc
- Pole position: Toni Elías, 2:09.062
- Fastest lap: Jaroslav Huleš, 2:09.648 on lap 8
- Podium: 1) Toni Elías 2) Lucio Cecchinello 3) Steve Jenkner

This race was notable for being the final podium for Àlex Crivillé, the 1999 500cc world champion. Standings for the top five riders and constructors after round ten were reported.