Glycoside hydrolase family 53

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Glycoside hydrolase family 53 (GH53) is a group of enzymes that break glycosidic bonds in carbohydrates. The main activity is beta-1,4-galactanase (EC 3.2.1.89), which cleaves beta-1,4 linkages in galactan, a component of plant cell walls. Enzymes in this family are classified by sequence similarity into many glycoside hydrolase families, with GH53 being one of them in databases such as CAZy and CAZypedia. GH53 enzymes typically fold into a TIM barrel structure, a common protein shape. They have been studied in organisms like Aspergillus aculeatus, and several protein structures exist in public databases.