HCT116 cells

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HCT116 is a human colon cancer cell line used to study cancer growth and test drugs. It has a KRAS mutation at codon 13 and an epithelial shape. The cells can form tumors in mice (xenografts) and can spread in that model. When the p53 gene is delivered by a virus, these cells stop dividing in the G1 phase. Growth of HCT116 cells can be slowed by 5-FU with P85 copolymer micelles. Knocking out MARCH2 also reduces their growth by causing stress in the endoplasmic reticulum. HCT116 cells are used to study colon cancer biology and the effects of various inhibitors. Cyclin D1 is important for lithocholic acid hydroxyamide activity in these cells. In xenograft studies, docetaxel, 5-FU, and flavopiridol can reduce tumor growth. There are two variants of HCT116: one with high Insp8 expression and one without; Insp8 is involved in energy metabolism and can influence cell behavior.